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Modifying Anthocyanins Biosynthesis in Tomato Hairy Roots: A Test Bed for Plant Resistance to Ionizing Radiation and Antioxidant Properties in Space

TitleModifying Anthocyanins Biosynthesis in Tomato Hairy Roots: A Test Bed for Plant Resistance to Ionizing Radiation and Antioxidant Properties in Space
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2022
AuthorsMassa, Silvia, Pagliarello Riccardo, Cemmi Alessia, Di Sarcina Ilaria, Bombarely Aureliano, Demurtas Olivia Costantina, Diretto Gianfranco, Paolini Francesca, H. Petzold Earl, Bliek Mattijs, Bennici Elisabetta, Del Fiore Antonella, De Rossi Patrizia, Spelt Cornelis, Koes Ronald, Quattrocchio Francesca, and Benvenuto Eugenio
JournalFrontiers in Plant Science
Volume13
Date PublishedDec-02-2023
ISSN1664462X
Abstract

Gene expression manipulation of specific metabolic pathways can be used to obtain bioaccumulation of valuable molecules and desired quality traits in plants. A single-gene approach to impact different traits would be greatly desirable in agrospace applications, where several aspects of plant physiology can be affected, influencing growth. In this work, MicroTom hairy root cultures expressing a MYB-like transcription factor that regulates the biosynthesis of anthocyanins in Petunia hybrida (PhAN4), were considered as a testbed for bio-fortified tomato whole plants aimed at agrospace applications. Ectopic expression of PhAN4 promoted biosynthesis of anthocyanins, allowing to profile 5 major derivatives of delphinidin and petunidin together with pelargonidin and malvidin-based anthocyanins, unusual in tomato. Consistent with PhAN4 features, transcriptomic profiling indicated upregulation of genes correlated to anthocyanin biosynthesis. Interestingly, a transcriptome reprogramming oriented to positive regulation of cell response to biotic, abiotic, and redox stimuli was evidenced. PhAN4 hairy root cultures showed the significant capability to counteract reactive oxygen species (ROS) accumulation and protein misfolding upon high-dose gamma irradiation, which is among the most potent pro-oxidant stress that can be encountered in space. These results may have significance in the engineering of whole tomato plants that can benefit space agriculture. Copyright © 2022 Massa, Pagliarello, Cemmi, Di Sarcina, Bombarely, Demurtas, Diretto, Paolini, Petzold, Bliek, Bennici, Del Fiore, De Rossi, Spelt, Koes, Quattrocchio and Benvenuto.

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URLhttps://www.frontiersin.org/articles/10.3389/fpls.2022.830931/full
DOI10.3389/fpls.2022.830931
Short TitleFront. Plant Sci.
Citation Key9675