| Title | Season linked responses to fine and quasi-ultrafine Milan PM in cultured cells |
|---|---|
| Publication Type | Articolo su Rivista peer-reviewed |
| Year of Publication | 2013 |
| Authors | Longhin, E., Pezzolato E., Mantecca P., Holme J.A., Franzetti A., Camatini M., and Gualtieri Maurizio |
| Journal | Toxicology in Vitro |
| Volume | 27 |
| Pagination | 551-559 |
| ISSN | 08872333 |
| Keywords | Air Pollutants, article, cell culture, Cell Line, Cell Survival, Chemical composition, coculture, Coculture Techniques, controlled study, Cytokines, DNA, DNA damage, double stranded DNA break, Endotoxins, histone H2AX, human, human cell, Humans, Italy, L-Lactate Dehydrogenase, lung alveolus cell, monocyte, particle size, particulate matter, protein phosphorylation, reactive oxygen metabolite, Reactive Oxygen Species, seasonal variation, Seasons, summer, Tumor, winter |
| Abstract | Exposure to urbane airborne particulate matter (PM) is related to the onset and exacerbation of cardiovascular and respiratory diseases. The fine (PM1), and quasi-ultrafine (PM0.4) Milan particles collected during different seasons have been characterised and the biological effects on human epithelial lung A549, monocytes THP-1 cells and their co-culture, evaluated and compared with the results obtained on the PM10 and PM2.5 fractions. Chemical composition and transmission electron microscopy (TEM) analysis of PM0.4 showed that this fraction was very similar to PM1 for biological responses and dimension. All the winter fractions increased within 1. h the level of reactive oxygen species (ROS), while only summer PM2.5 had this effect on A549 cells. The phosphorylation of H2AX (γH2AX), a marker of double strand DNA breaks (DSBs), was increased by all the winter fractions on A549 and THP-1 cells while summer PM samples did not induced this effect. PM0.4 and PM1 biological effects are partly similar and related to the season of sampling, with effects on ROS and DNA damage induced only by winter PM fractions. The winter PM damaging effect on DNA correlates with the presence of organic compounds. © 2012 Elsevier Ltd. |
| Notes | cited By 22 |
| URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84874603426&doi=10.1016%2fj.tiv.2012.10.018&partnerID=40&md5=34b07f04200bd952afa5104b8ca10634 |
| DOI | 10.1016/j.tiv.2012.10.018 |
| Citation Key | Longhin2013551 |
