Sorry, you need to enable JavaScript to visit this website.

Physical profile and impact of a calcium-incorporated implant surface on preosteoblastic cell morphologic and differentiation parameters: A comparative analysis

TitlePhysical profile and impact of a calcium-incorporated implant surface on preosteoblastic cell morphologic and differentiation parameters: A comparative analysis
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2016
AuthorsLollobrigida, M., Lamazza L., Capuano C., Formisano G., Serra Emanuele, Laurito D., Romanelli M., Molinari A., and de Biase A.
JournalInternational Journal of Oral and Maxillofacial Implants
Volume31
Pagination223-231
ISSN08822786
Keywords3T3 cell line, 3T3 Cells, Acid Etching, alkaline phosphatase, animal, Animals, Calcium, cell culture technique, Cell Culture Techniques, cell differentiation, cell proliferation, cell shape, chemistry, comparative study, cytology, Dental, dental acid etching, dental etching, dental material, Dental Materials, electron, Interferometry, light, Materials testing, Mice, Microscopy, mouse, Nanomaterial, Nanostructures, osteoblast, Osteoblasts, osteocalcin, physiology, procedures, Scanning, Scanning electron microscopy, Surface properties, surface property, time factor, Time Factors, Titanium
Abstract

Purpose: To assess and compare topographic features and preosteoblastic cell responses of a new hydrothermally treated, calcium-incorporated surface against other commercially available implant surfaces. Materials and Methods: Four different surfaces were the subject of comparison in this study: machined (MC), resorbable blast media (RBM), sandblasted/large-grit/acid-etched (SLA), and calciumincorporated SLA (Ca-SLA). Surface morphology and roughness were first characterized by scanning electron microscope (SEM) and white light interferometer, respectively. Preosteoblastic MC3T3-E1 cells were then cultured on the titanium surfaces. Cell morphology was observed at 24 hours, 48 hours, 7 days, and 15 days by SEM; differentiation was assessed at 7, 11, and 15 days by assaying alkaline phosphatase (ALP) activity and osteocalcin (OCN) levels. Results: Surface characterization revealed nanotopographic features on Ca-SLA. At topographic analysis, SLA and Ca-SLA showed similar roughness values. Significant differences in cell differentiation parameters were found only at 15 days between the SLA surfaces (both Ca-incorporated and nonincorporated) and MC. Conclusion: Collectively, this study demonstrated that hydrothermal treatment determines the formation of nanotopography without altering the SLA microtopography. Moreover, Ca-SLA and SLA induce MC3T3-E1 cell differentiation at comparable levels. © 2016 by Quintessence Publishing Co Inc.

Notes

cited By 0

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84979093811&doi=10.11607%2fjomi.4247&partnerID=40&md5=d8dc96be128e0261736d07275be0cbf6
DOI10.11607/jomi.4247
Citation KeyLollobrigida2016223