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Sea urchin embryotoxicity test: Proposal for a simplified bioassay

TitleSea urchin embryotoxicity test: Proposal for a simplified bioassay
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2004
AuthorsManzo, Sonia
JournalEcotoxicology and Environmental Safety
Volume57
Pagination123-128
ISSN01476513
KeywordsAnimals, article, Bioassay, Biological Assay, calculation, Chromium, controlled study, copper sulfate, Correlation analysis, dichromate potassium, Echinoidea, ecotoxicology, Embryo, embryotoxicity, experimentation, filtration, Heavy, heavy metal, laboratory, Lethal Dose 50, marine environment, Metals, nonhuman, Paracentrotus lividus, Reliability, Reproducibility of Results, Sea urchin, Sea Urchins, sea water, statistical significance, Toxicity, toxicity testing, Toxicity Tests, Water Pollutants, Zygote
Abstract

{Sea urchin embryotoxicity tests are widely used for evaluating the biological effects of contaminants in marine environments. The currently used traditional and standardized protocols are quite slow and laborious. The present work shows a modified bioassay (new embryotoxicity test; NET) in an attempt to speed up laboratory work using a limited number of fertilized eggs. Several experiments have been conducted both with a traditional bioassay and with the NET, using the same test conditions, in order to evaluate the reliability of the proposed simplified bioassay. Adult Paracentrotus lividus (Lamark) were collected from the Tyrrenian Sea (Bay of Naples) and embryos, reared in filtered seawater, were exposed to increasing potassium dichromate and copper sulfate concentrations. Then the EC50 was calculated. The analysis of the results evidenced good repeatability. The confidence limits in all tests overlapped; moreover, data correlation analysis between the results of both tests showed a high significant accordance (chromium, R 2=0.93, P<0.01; copper

Notes

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0842264832&doi=10.1016%2fj.ecoenv.2003.10.007&partnerID=40&md5=cecff4c2335887b85e5d0c2bd9de2b59
DOI10.1016/j.ecoenv.2003.10.007
Citation KeyManzo2004123