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Effect of layered double hydroxide intercalated with fluoride ions on the physical, biological and release properties of a dental composite resin

TitleEffect of layered double hydroxide intercalated with fluoride ions on the physical, biological and release properties of a dental composite resin
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2014
AuthorsTammaro, Loredana, Vittoria V., Calarco A., Petillo O., Riccitiello F., and Peluso G.
JournalJournal of Dentistry
Volume42
Pagination60-67
ISSN03005712
Keywordsadolescent, alkaline phosphatase, aluminum hydroxide, anticaries agent, bisphenol A bis(2 hydroxypropyl) ether dimethacrylate, Bisphenol A-Glycidyl Methacrylate, Cariostatic Agents, cell culture, cell differentiation, cell proliferation, Cells, chemistry, Composite Resins, Cultured, cytology, delayed release formulation, Delayed-Action Preparations, dental material, Dental Materials, Dental Pulp, diffusion, drug effects, ethoxylated bis-phenol A dimethacrylate, fluoride, Fluorides, Fourier Transform Infrared, human, Humans, hydrotalcite, hydroxide, hydroxide ion, Hydroxides, infrared spectroscopy, Ion exchange, macrogol derivative, magnesium hydroxide, Mechanical, mechanical stress, Methacrylates, methacrylic acid derivative, Polyethylene Glycols, polymethacrylic acid derivative, Polymethacrylic Acids, resin, Spectroscopy, stem cell, Stem cells, stress, Temperature, tooth pulp, triethylene glycol dimethacrylate, X ray diffraction, X-Ray Diffraction, young adult
Abstract

Objectives The aim of this work was the preparation of a new fluoride-releasing dental material characterized by a release of fluoride relatively constant over time without any initial toxic burst effect. This type of delivery is obtained by a matrix controlled elution and elicits the beneficial effect of a low amount of fluoride on human dental pulp stem cells (hDPSCs) towards mature phenotype. Methods The modified hydrotalcite intercalated with fluoride ions (LDH-F), used as filler, was prepared via ion exchange procedure and characterized by X-ray diffraction and FT-IR spectroscopy. The LDH-F inorganic particles (0.7, 5, 10, 20 wt.%) were mixed with a photo-activated Bis-GMA/TEGDMA (45/55 wt/wt) matrix and novel visible-light cured composites were prepared. The dynamic thermo-mechanical properties were determined by dynamic mechanical analyzer. The release of fluoride ions in physiological solution was determined using a ionometer. Total DNA content was measured by a PicoGreen dsDNA quantification kit to assess the proliferation rate of hDPSCs. Alkaline phosphatase activity (ALP) was measured in presence of fluoride resins. Results Incorporation of even small mass fractions (e.g. 0.7 and 5 wt.%) of the fluoride LDH in Bis-GMA/TEGDMA dental resin significantly improved the mechanical properties of the pristine resin, in particular at 37 C. The observed reinforcement increases on increasing the filler concentration. The release of fluoride ions resulted very slow, lasting months. ALP activity gradually increased for 28 days in hDPSCs cell grown, demonstrating that low concentrations of fluoride contributed to the cell differentiation. Conclusions The prepared composites containing different amount of hydrotalcite filler showed improved mechanical properties, slow fluoride release and promoted hDPSCs cell proliferation and cell differentiation. © 2013 Published by Elsevier Ltd.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84890982649&doi=10.1016%2fj.jdent.2013.10.019&partnerID=40&md5=61aae489d2397dd5180dfa062f24b16f
DOI10.1016/j.jdent.2013.10.019
Citation KeyTammaro201460