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Differential effects of gonadectomy on thymic stromal cells in promoting T cell differentiation in mice

TitleDifferential effects of gonadectomy on thymic stromal cells in promoting T cell differentiation in mice
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication1995
AuthorsUtsuyama, M., Hirokawa K., Mancini C., Brunelli R., Leter Giorgio, and Doria G.
JournalMechanisms of Ageing and Development
Volume81
Pagination107-117
ISSN00476374
KeywordsAging, animal, animal experiment, animal tissue, Animalia, Animals, article, CD4-CD8 Ratio, Cell Count, cell differentiation, cell population, cell proliferation, comparative study, concanavalin a, controlled study, Evaluation Studies, Flow cytometry, gonadectomy, Inbred C57BL, Inbred DBA, leukocyte count, lymphocyte differentiation, male, Mice, mouse, newborn, nonhuman, Orchiectomy, organ growth, precursor, priority journal, stroma cell, Stromal Cells, T lymphocyte, T-Lymphocytes, testis, thymocyte, thymus, Thymus Gland, thymus graft, Thymus Hyperplasia
Abstract

Twenty-six week-old BDF1 mice were gonadectomized and grafted with thymus from irradiated (8.5 Gy) newborn, 6-week-old, or 26-week-old mice. One month later, grafted thymuses were recovered and examined in terms of thymocyte numbers, subpopulations and proliferative responses to Concananavlin A (Con A). The growth of the irradiated thymus was significantly higher in gonadectomized (Gx) than in sham-operated (Sham) mice and the magnitude of thymic growth was apparently age-dependent, as it was greater for newborns than for older mice. Con A response of thymocytes was also significantly higher in Gx mice than in Sham mice, and the magnitude of the response declined with advancing age of the thymus donors. Flow cytometric analysis revealed that a significant increase in the percentage of CD4+CD8- was observed in thymus grafts showing high Con A responses. However, this effect of Gx on the thymus graft was dependent on age of the thymus donor. Namely, newborn thymus grafts could grow equally well in both Gx and Sham recipients, whereas thymus grafts from 6- and 26-week-old mice could grow well only in Gx, but not in Sham recipients. The number of thymocytes was comparable in thymus grafts from 6- and 26-week-old mice, but the proliferative response to Con A was higher in the former than in the latter graft. Collectively, Gx appeared to promote immigration of thymocyte precursors into the thymus and to enhance proliferation and differentiation of thymocytes towards CD4+CD8- T cells, in an age-related manner. © 1995.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0029039784&doi=10.1016%2f0047-6374%2895%2901589-R&partnerID=40&md5=34a60baa220d872150028f2fb666c8f3
DOI10.1016/0047-6374(95)01589-R
Citation KeyUtsuyama1995107