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Different portions of the maize root system host Burkholderia cepacia populations with different degrees of genetic polymorphism

TitleDifferent portions of the maize root system host Burkholderia cepacia populations with different degrees of genetic polymorphism
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2000
AuthorsChiarini, L., Giovannelli V., Bevivino Annamaria, Dalmastri Claudia, and Tabacchioni Silvia
JournalEnvironmental Microbiology
Volume2
Pagination111 - 118
Date Published2000///
Abstract

In order to acquire a better understanding of the spatial and temporal variations of genetic diversity of Burkholderia cepacia populations in the rhizosphere of Zea mays, 161 strains were isolated from three portions of the maize root system at different soil depths and at three distinct plant growth stages. The genetic diversity among B. cepacia isolates was analysed by means of the random amplified polymorphic DNA (RAPD) technique. A number of diversity indices (richness, Shannon diversity, evenness and mean genetic distance) were calculated for each bacterial population isolated from the different root system portions. Moreover, the analysis of molecular variance (AMOVA) method was applied to estimate the genetic differences among the various bacterial populations. Our results showed that, in young plants, B. cepacia colonized preferentially the upper part of the root system, whereas in mature plants, B. cepacia was mostly recovered from the terminal part of the root system. This uneven distribution of B. cepacia cells among different root system portions partially reflected marked genetic differences among the B. cepacia populations isolated along maize roots on three distinct sampling occasions. In fact, all the diversity indices calculated indicated that genetic diversity increased during plant development and that the highest diversity values were found in mature maize plants, in particular in the middle and terminal portions of the root system. Moreover, the analysis of RAPD patterns by means of the AMOVA method revealed highly significant divergences in the degree of genetic polymorphism among the various B. cepacia populations.

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Cited By (since 1996): 15Export Date: 26 August 2010Source: Scopus

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0034544409&partnerID=40&md5=a6d8b66d8600205fea79010a5d7ea598
Citation Key411