A pilot-scale moving-bed biofilm reactor (MBBR), operated under alternating intermittent aeration (IA) and non-aeration phases, was used for single-stage carbon (C) and nitrogen (N) removal from high-fluctuating municipal wastewater via simultaneous nitrification–denitrification. The reactor was operated under highly variable chemical oxygen demand to total nitrogen (COD/TN) ratios, low dissolved oxygen (DO) conditions, and progressively extended non-aerated periods to evaluate process robustness under real operational conditions. An active denitrifying biofilm developed on the carriers after 23 days of the anoxic start-up, as confirmed by batch activity tests. Under the most carbon-limited condition tested (COD/TN = 5.5), the application of 16 h·d⁻¹ of non-aerated phases at DO levels of 0–1.0 mg·L⁻¹ enabled simultaneous COD, N–NH₄⁺ and TN removal efficiencies of 70, 95 and 84%, respectively. These results confirm that transient IA is an effective strategy for simultaneous C and N removal at very low COD/TN ratios and real fluctuating influent concentrations. Energy assessment showed that extended non-aeration phases reduced blower energy demand by 67% and total plant energy consumption by 34%, improving the environmental sustainability of the single-stage process. The main novelty of this study lies in the pilot-scale validation of an IA-MBBR for SND using real municipal wastewater under naturally fluctuating C and N loadings, thereby bridging previous laboratory-scale evidence with realistic operating conditions. © 2026 by the authors.

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