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Ex-situ bioremediation of petroleum hydrocarbon contaminated soil using mixed stimulants: Response and dynamics of bacterial community and phytotoxicity

TitoloEx-situ bioremediation of petroleum hydrocarbon contaminated soil using mixed stimulants: Response and dynamics of bacterial community and phytotoxicity
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2022
AutoriAmbaye, T.G., Chebbi A., Formicola F., Rosatelli A., Prasad S., Gomez F.H., Sbaffoni Silvia, Franzetti A., and Vaccari M.
RivistaJournal of Environmental Chemical Engineering
Volume10
ISSN22133437
Parole chiave% reductions, activated sludge, Arthrobacter, Bacteria, Bacterial community, Bacteriology, Bio-surfactants, Biodegradation, Biomolecules, Bioremediation, Biotechnology, Contaminated soils, Ex situ bioremediation, gasoline, Hydrocarbons, Mesocosms, Nutrients, Petroleum chemistry, phytotoxicity, RNA, Soil pollution, Soils, Stimulant, Surface active agents
Abstract

Pollution of agricultural soils by excessive petroleum hydrocarbons (PHs) has recently become a severe worldwide environmental issue. Various approaches have been recently employed to biodegrade PHs and detoxify soils. In the present study, an experimental mesocosm was developed in a bioslurry reactor. Six treatments were applied to degrade PHs-and detoxify contaminated soil by adding external stimulants such as nutrients, activated sludge, synthetic surfactant, and long-chain rhamnolipids. The comparative analysis revealed that the combination of activated sludge, nutrients, and biosurfactant showed a 79.4% reduction in TPHs after 10 days in the mesocosm experiment. The lowest reduction in total PHs (48.5 %) was attained in the nutrient-supplied mesocosms. The consortia's kinetic analyses of PHs revealed the lowest half-life time (t1/2 = 4.1 days), the highest biodegradation rate constants (k = 0.17 day1), and the reaction rate data from each bioremediation method was fitted with a first-order reaction rate model. The 16S rRNA gene amplicon and sequencing revealed that Streptomyces, Nocardioides, Arthrobacter, Pseudomonas, and Bacillus were the main oxidative species as PHs degraders. The highest relative abundances were in Arthrobacter, Pseudomonas, and Bacillus. The phytotoxicity studies proved that there was a significant decrease in the toxicity of soil after the biotreatment. According to the findings of this study, adding external stimulants such as activated sludge, nutrients, and biosurfactants enhanced the degradation of PHs in the soil in bioslurry enhancement as a promising approach to pilot scale. © 2022 Elsevier Ltd.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85141934707&doi=10.1016%2fj.jece.2022.108814&partnerID=40&md5=e0675fee83aa218f08aba41391f755e2
DOI10.1016/j.jece.2022.108814
Citation KeyAmbaye2022