| Titolo | Self-assembled extracellular matrix protein networks by microcontact printing |
|---|---|
| Tipo di pubblicazione | Articolo su Rivista peer-reviewed |
| Anno di Pubblicazione | 2004 |
| Autori | Sgarbi, N., Pisignano D., Di Benedetto Francesca, Gigli G., Cingolani R., and Rinaldi R. |
| Rivista | Biomaterials |
| Volume | 25 |
| Paginazione | 1349-1353 |
| ISSN | 01429612 |
| Parole chiave | antigenicity, article, Atomic force microscopy, Bioassay, Biocompatible, cell culture, Coated materials, Crystallization, Extracellular Matrix Proteins, glass, Immunofluorescence, immunology, Laminin, laminin 1, Materials testing, physiology, priority journal, protein assembly, Protein Conformation, protein determination, Proteins, scleroprotein, Self assembly, structure analysis, Substrates, Supramolecular chemistry, tissue engineering |
| Abstract | Physiological patterns of the extracellular matrix protein, laminin-1, were obtained on glass substrates by physisorption-assisted microcontact printing. Besides the well-retained antigenicity confirmed by indirect immunofluorescence assays, we investigated the supramolecular organization of the proteins by atomic force microscopy. We found the characteristic protein self-assembling in polygonal networks with well-defined sub-100nm quaternary structures of laminin. The formation of these physiological mesh-like protein matrices was obtained by means of one-step soft lithography without any preliminary functionalization of glass, which can be exploited for many possible applications for cell cultures and biomolecular devices. © 2003 Elsevier Ltd. All rights reserved. |
| Note | cited By 39 |
| URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0345256508&doi=10.1016%2fj.biomaterials.2003.08.017&partnerID=40&md5=44dcdd9303b8b13786cabb7645a16cf8 |
| DOI | 10.1016/j.biomaterials.2003.08.017 |
| Citation Key | Sgarbi20041349 |
