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Comparison of 7,12-Dimethylbenz(a)anthracene-DNA Adduction in the Epidermis of Two Lines of Mice Selected for Resistance (CAR-R) or Susceptibility (CAR-S) to Skin Carcinogenesis

TitoloComparison of 7,12-Dimethylbenz(a)anthracene-DNA Adduction in the Epidermis of Two Lines of Mice Selected for Resistance (CAR-R) or Susceptibility (CAR-S) to Skin Carcinogenesis
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione1994
AutoriPerin, F., Perin O., Barat N., Plessis M.J., Saran Anna, Pioli Claudio, Covelli V., Biozzi G., and Mouton D.
RivistaCancer Research
Volume54
Paginazione4635-4640
ISSN00085472
Parole chiave10-Dimethyl-1, 2-benzanthracene, 9, animal cell, animal model, animal tissue, Animals, article, cancer resistance, cancer susceptibility, Cell Line, chemical carcinogenesis, controlled study, dimethylbenz[a]anthracene, DNA, dna adduct, DNA Adducts, dna drug complex, Drug Resistance, Epidermis, Female, Gene expression, Mice, mouse, nonhuman, phorbol 13 acetate 12 myristate, priority journal, quantitative assay, quantitative genetics, Skin, skin carcinogenesis, Skin Neoplasms, strain difference, Tetradecanoylphorbol Acetate, topical drug administration
Abstract

Two lines of mice were produced by bidirectional selective breeding: one resistant (CAR-R) and one susceptible (CAR-S) to two-stage skin carcinogenesis by dimethylbenz(a)anthracene and 12-O-tetradecanoylphorbol-13-acetate. The dimethylbenz(a)anthracene-DNA adduct formation was compared in the two lines by a postlabeling procedure so as to determine whether the striking interline difference observed as to tumor incidence could (in part) be due to differences in the formation of DNA-reactive metabolites. Results show that qualitatively, adduct profiles in CAR-R and CAR-S epidermis are similar. Quantitatively, the total binding level is slightly higher in CAR-S versus CAR-R mice during the 30-day follow-up. However, these minor differences do not increase in function of the response to selection observed through three consecutive generations. A 2- or 4-week promotion with 12-0-tetradecanoylphorbol-13-acetate enhances the decrease of adduct level in the two lines. This effect is somewhat more pronounced in CAR-S mice. Results strongly suggest that the expression of the genes responsible for CAR-R/CAR-S phenotypic difference affects mainly the postinitiation stages. © 1994, American Association for Cancer Research. All rights reserved.

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Citation KeyPerin19944635