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Chemical characterization and surface properties of a new bioemulsifier produced by Pedobacter sp. strain MCC-Z

TitoloChemical characterization and surface properties of a new bioemulsifier produced by Pedobacter sp. strain MCC-Z
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2015
AutoriBeltrani, Tiziana, Chiavarini Salvatore, Cicero D.O., Grimaldi M., Ruggeri C., Tamburini E., and Cremisini C.
RivistaInternational Journal of Biological Macromolecules
Parole chiavealiphatic hydrocarbon, aromatic hydrocarbon, article, bacterial strain, bacterium culture, carbohydrate derivative, Chemical analysis, Chemical composition, chemical phenomena, chemistry, complex formation, controlled study, critical micelle concentration, diesel fuel, diffusion, emulsifying agent, Emulsifying Agents, emulsion, enzyme specificity, fatty acid, Fatty acids, Fourier Transform Infrared, galactose, high performance liquid chromatography, hydrodynamics, Hydrophobic and Hydrophilic Interactions, infrared spectroscopy, isolation and purification, mass fragmentography, Molecular Weight, monosaccharide, Monosaccharides, Pedobacter, pentadecanoic acid, Proton Magnetic Resonance Spectroscopy, proton nuclear magnetic resonance, Purification, Spectroscopy, structure analysis, surface property, surface tension, surfactant, talose, unclassified drug, Water

A novel biopolymer was described in the form of an extracellular polysaccharide (EPS) by Pedobacter sp. strain MCC-Z, a member of a bacterial genus not previously described as an emulsifier producer. The new biomolecule was extracted, purified and characterized, and its surface and emulsifying properties were evaluated. The purified bioemulsifier, named Pdb-Z, showed high emulsifying activity (E24%=64%) and reduced the surface tension of water up to 41mN/m with a critical micelle concentration value of 2.6mg/mL. The chemical characterization of Pdb-Z was performed using 1H NMR, FT-IR, HPLC/MS/MS and GC/MS. Pdb-Z was found to contain 67% of carbohydrates, consisting mainly of galactose and minor quantities of talose, 30% of lipids, being pentadecanoic acid the major lipidic constituent, and 3% of proteins. The bioemulsifier was a glycolipids-protein complex with an estimated molecular mass of 106Da. Furthermore, Pdb-Z emulsified pure aliphatic and aromatic hydrocarbons as well as diesel more efficiently than commercial synthetic surfactants, used for comparison. Our results suggest Pdb-Z has interesting properties for applications in remediation of hydrocarbon-contaminated environments and bioremediation processes. © 2014 Elsevier B.V..


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