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Interaction of lactoferrin with Escherichia coli cells and correlation with antibacterial activity

TitoloInteraction of lactoferrin with Escherichia coli cells and correlation with antibacterial activity
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione1990
AutoriVisca, P., Dalmastri Claudia, Verzili D., Antonini G., Chiancone E., and Valenti P.
RivistaMedical Microbiology and Immunology
Volume179
Paginazione323 - 333
Data di pubblicazione1990///
Abstract

It has been established that the antimicrobial activity of lactoferrin towards Escherichia coli is enhanced by a direct contact between the protein and the microbial cell and that, in the case of E. coli K-12 strains, an antibacterial activity of lactoferrin unrelated to iron withdrawal is present. Evidence is now reported that lactoferrin binds to surface structures expressed in E. coli K-12 strains grown in either an ’excess’ or ’stress’ of iron. Under the experimental conditions used, lactoferrin binding both in the apo and in the iron-saturated form yields a maximum of 1.6 x 105 bound molecules/E. coli K-12 cell; the amount of lactoferrin bound does not depend on the expression of the iron-regulated outer membrane proteins. In contrast, lactoferrin does not bind to E. coli clinical isolates. Apo-lactoferrin (at 500 μg/ml in a chemically defined medium) inhibits the growth of E. coli K-12 strains but not of clinical isolates. These findings suggest that the antibacterial activity of the protein could be associated to its binding to the cell surface.

Note

Cited By (since 1996): 16Export Date: 26 August 2010Source: Scopus

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0025659446&partnerID=40&md5=ade44900d6369a9fbbf07019b2da689b
Citation Key489