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Epigenetic-based antioxidant effect of an ethanolic extract of Corylus avellana L. on THLE-2 human primary hepatocytes

TitoloEpigenetic-based antioxidant effect of an ethanolic extract of Corylus avellana L. on THLE-2 human primary hepatocytes
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2023
AutoriBenassi, Barbara, Bacchetta Loretta, and Pacchierotti Francesca
RivistaNatural Product Research
Paginazione1 - 7
Data di pubblicazioneMar-02-2023
ISSN14786419
Abstract

The ethanolic extract of Corylus avellana L hazelnut, prepared in our laboratories, has been previously characterized by liquid chromatography coupled to high resolution mass spectrometry. We here aimed at testing the antioxidant effect of such extract in H2O2-challenged THLE-2 human primary hepatocytes and verified whether it might be based on microRNA-34b/c expression changes. We here demonstrate that miR-34b/miR-34c undergo significant stimulation (≥2-fold change, p < 0.05) in THLE-2 when treated for 72h with not-toxic hazelnut concentrations (0.04–0.4 mg/ml), when compared with 0.06% ethanol control. When administered with H2O2 (1000–2000 µM, 24h), THLE-2 are significantly protected from oxidative stress if pre-treated with hazelnut, the H2O2-driven cytotoxicity and reactive oxygen species generation being recovered by hazelnut extract, through miR-34b/c stimulation. Although preliminary, our findings pave the way for further preclinical studies aimed at validating the possible health-related application of hazelnut matrix, and/or its metabolites, as powerful epigenetic-based drugs, food supplements or nutraceuticals. © 2023 Informa UK Limited, trading as Taylor & Francis Group.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85147671292&doi=10.1080%2f14786419.2023.2174537&partnerID=40&md5=49f830f89e30329c2797b5ddd92d02d8
DOI10.1080/14786419.2023.2174537
Titolo breveNatural Product Research
Citation KeyBenassi2023